Journal: bioRxiv
Article Title: Tracking eco-evolutionary dynamics among lung pathobiome members from children with cystic fibrosis
doi: 10.64898/2026.01.29.702708
Figure Lengend Snippet: (A) Phylogenetic tree based on 1196 core genes of clinical H. influenzae isolates and three well-studied laboratory strains (86-028NP, ATCC 10211, and ATCC 49247). The table displays the ID of people with CF (pwCF) and the capsule presence (typeable vs non-typeable). Note that one isolate had to be excluded from genetic analysis due to poor sequence qualities. (B) Genome size (in base pairs) of the clinical isolates, grouped by time window. (C) Average genome composition of clinical isolates, with genes classified as core (present in 100% of isolates), soft-core (90–99%), shell (15–89%), and cloud (0–14%). (D) Protease activity of clinical isolates measured using the azocasein assay. (E) Siderophore production of clinical isolates measured using the chrome azurol S (CAS) assay in ssBHI medium supplemented with 2,2’-bipyridine to a final concentration of 75 μM. (F) Biofilm formation under static growth conditions measured using the crystal violet assay. Asterisks denote significance levels: ***P < 0.001; **P < 0.01; *P < 0.05.
Article Snippet: Furthermore, we used laboratory strains, including P. aeruginosa PAO1 (ATCC 15692), S. aureus strains USA300 JE2 (ATCC-BAA-1717), Cowan 1 (ATCC 12598), and 6850 (ATCC 53657), and H. influenzae strains ATCC 49766 (NTHI), ATCC 49247 (NTHI) and ATCC 10211 (Serovar B) (Table S4).
Techniques: Sequencing, Activity Assay, Azocasein Assay, Concentration Assay, Crystal Violet Assay